PROJECT INFORMATION
GOLD Project ID
Gp0099196  
Project Name
Penicillium chrysogenum UCB PC0814C  
Other Names
  
Legacy ER Project ID
76055  
Legacy GOLD ID
Gi0076055  
NCBI BioProject Name Penicillium chrysogenum strain:PC0814C
NCBI BioProject ID 243542
NCBI BioProject Accession PRJNA243542
NCBI Locus Tag
DK99  
NCBI BioSample Accession
PI
Added By JGI automated process on 2014-07-18
Last Modified By
Project Comments
  
Project Status
incomplete  
Project Relevance Evolution
Sequencing Center University of California, Berkeley (USA)
Collaborating Institute
  
Funding Agency
  
Project Description
Strain selection and strain improvement are the first, and arguably most important, steps in the industrial production of biological compounds by microorganisms. While traditional methods of mutagenesis and selection have been effective in improving production of compounds at a commercial scale, the genetic changes underpinning the altered phenotypes have remained largely unclear. We utilized high-throughput Illumina short read sequencing of a wild Penicillium chrysogenum strain in order to make whole genome comparisons to a sequenced improved strain (WIS 54-1255). We developed an assembly-free method of identifying chromosomal rearrangements and validated the in silico predictions with a PCR-based assay and Sanger sequencing. Despite many rounds of mutagen treatment and artificial selection, WIS 54-1255 differs from its wild progenitor at only one of the identified rearrangements. We suggest that the identification of natural variants predisposed to be high penicillin producers was instrumental in the success of WIS 54-1255 as an industrial producer. In addition to finding a previously published inversion in the penicillin biosynthesis cluster, we located several genes related to penicillin production associated with these rearrangements. By comparing the configuration of rearrangement events among several historically important strains known to be high penicillin producers to a collection of recently isolated wild strains, we suggest that wild strains with rearrangements similar to those in known high penicillin producers may be viable candidates for further improvement efforts.  
Is JGI Project
No  
Project Information Visibility Public
PROJECT TYPE
Specimen Organism
Nucleic Acid DNA
Sequencing Strategy
Whole Genome Sequencing  
EXTERNAL PROJECT LINKS
Project Information
  
Database
  
Genome Publications
  
Other Publications
  
SRA Experiment
  
PROJECT COMPOSITION
Study Penicillium chrysogenum UCB strain PC0814C Genome sequencing
Organism Penicillium chrysogenum UCB PC0814C
Analysis Projects
  
Number of Analysis Projects (AP)
0  
Number of Derived Analyis Projects (DAP)
0  
SEQUENCING INFORMATION
Sequencing Status In progress
Sequencing Quality (MIGS-31)
Finishing Goal
  
Contamination Screening Input
  
DNA Amplification Method
  
Sequencing Comments
  
DNA Amplification Kit
  
Sequencing Technology (MIGS-29)
  
Library Method (MIGS-28)
  
Library Layout
  
Library Screening Method
  
Number Of Reads
  
Read Size
  
Vector
  
GC Percent
  
Chromosome Count
  
Plasmid Count
  
ORGANISM NAME
GOLD Organism ID Go0099196
Organism Name
Penicillium chrysogenum UCB PC0814C  
Other names (alias, synonyms, short names, common names)
  
Organism Domain
EUKARYAL  
Phylogeny
FUNGI-ASCOMYCOTA  
Genus
Penicillium  
Genus Synonyms
  
Species
Penicillium chrysogenum  
Subspecies
  
Species Synonyms
  
Strain
UCB PC0814C  
Strain Synonyms
  
Serovar/Cultivar
  
Culture Collection ID
  
Type Strain
Unknown  
Exemplar DOI
  
Exemplar Name
  
Taxon DOI
  
Biosafety Level
  
Organism Comments
  
Cultured
Yes  
Culture Type
Isolate  
Organism Type
Natural  
Uncultured Type
  
Commercial Strain
  
Commercial Strain Comments
  
ORGANISM TAXONOMY
NCBI Taxonomy ID 5076
NCBI Superkingdom
Eukaryota  
NCBI Kingdom
Fungi  
NCBI Phylum
Ascomycota  
NCBI Class
Eurotiomycetes  
NCBI Order
Eurotiales  
NCBI Family
Aspergillaceae  
NCBI Genus
Penicillium  
NCBI Species
Penicillium chrysogenum  
ISOLATION CLASSIFICATION
Ecosystem
  
Ecosystem Category
  
Ecosystem Type
  
Ecosystem Subtype
  
Specific Ecosystem
  
ORGANISM EXTERNAL REFERENCES
Strain Info ID
Genbank 16S ID
GENERAL PROPERTIES
Oxygen Requirement
  
Cell Shape
  
Motility
  
Sporulation
  
Temperature Range
  
Salinity
  
pH
  
Cell Diameter
  
Cell Length
  
Color
  
Gram Staining
  
Biotic Relationships
  
Symbiotic Physical Interaction
  
Symbiotic Relationship
  
Symbiont Name
  
Symbiont Taxon ID
  
Cell Arrangements
  
Diseases
  
Known Habitats
  
Metabolisms
  
Phenotypes
  
Energy Sources
  

 

 

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